Composite

Part:BBa_K5048061:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


T7 promoter-Neae-FLAG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2103
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
    Illegal XhoI site found at 2112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1343


Design Notes

We put the Neae gene under the regulation of the T7 promoter to ensure adequate expression of the surface display system. A FLAG tag was added for convenient detection by WB and immunofluorescence.


Source

See our part:BBa_K5048047


References